ACADEMY OF ROMANIAN SCIENTISTS ANNALS
Series on BIOLOGICAL SCIENCES
VOLUME 8 2019 NUMBER 2
ISSN 2285 – 4169
TOPICS:
• Anthropology
• Biochemistry
• Biodiversity and nature conservation
• Biology of development
• Biomedicine
• Biotechnology
• Cell and molecular biology
• Ecology
• Genetics and bioevolution
• Marine biology
• Microbiology
• Neurosciences
• Plant and animal physiology
• Pharmacology
Editura
Academiei Oamenilor de Ştiinţă din România Bucureşti, 2019
Natural Modulation of Intestinal and Cutanate Microbiome
in Patients with Psoriasis
Gheorghe GIURGIU1, Manole COJOCARU2
1 Deniplant Aide-Sante Biomedicine Center, Bucharest
2 Titu Maiorescu University, Faculty of Medicine, Bucharest
* author correspondent (e-mail) :
deniplant@gmail.com
Abstract
Through the modulatory effect on the body's immune system, the
intestinal and cutaneous microbiome exert influences on the skin
homeostasis. The microbiome exhibits beneficial actions in the body
under conditions of eubiosis, however, when dysbiosis occurs, the
microbiome can trigger various autoimmune, metabolic, neurological
conditions (eg allergies, eczema, asthma, psoriasis, diabetes, central
nervous disorders). This can happen when fragments of microbial DNA
cross the intestinal barrier and reach the bloodstream and even into
the nervous system. Although modern medicine is trying to modulate the
microbiome, to use it for the treatment of certain conditions, this
has not been achieved so far. By using antibiotics and other
medicines, the microbiome can be severely affected by affecting the
health of the body. In the last five years, discoveries in the field
of genetics and immunology have allowed the initiation of new studies
on the role of the intestinal and skin microbiome in patients with
psoriasis. Using natural herbal remedies and fruit tree buds, we
developed a natural modulator of the intestinal and cutaneous
microbiome with which it was able to slow down and even stop the
evolution of psoriasis, without ointments or other drugs and without
food restrictions. Thus, the path has been opened to the realization
of functional foods (nutraceuticals) with dual role: nutrition and
health, which can naturally modulate the activity of the human
microbiome, restore the eubiosis, the processes of cell restoration
and healing of the organism.
Key words: microbiome, psoriasis, autoimmune diseases, natural
remedies, functional foods (nutraceuticals).
Introduction
(1) The paper presents new ways of preventing and treating psoriasis
disease through the natural modulation of the intestinal and skin
microbiome.
(2) Although psoriasis is a disease that has been present for hundreds
of years, dermatology treats it as a strict skin condition and for
this reason the treatments are generally aimed at healing the surface
skin. For this reason the cure of the disease is only partial, with
multiple relapses and extensions. Although we are trying to find an
effective treatment of internal causes, so far this has not been
achieved and for this reason the psoriasis disease is classified as
incurable.
(3) With the help of phytotherapy and gemotherapy, respectively with
medicinal plants and fruit buds, acting on the dysbiosis of the
intestinal and skin microbiome, it can intervene on the immune system
and the process of apoptosis of the epithelial cells and by triggering
self-healing reactions in the body, psoriatic lesions disappear by
themselves.
(4) During the last five years the discoveries in the genetic and
immunological field, have allowed the initiation of new studies
regarding the role played by the skin and intestinal microbiome in the
patients with psoriasis. Thus, Golden Staphylococcus or Pyogenes
Streptococcus may induce maintenance or exacerbation of psoriasis
lesions. An important role in the occurrence and maintenance of
psoriasis lesions is also the microbial flora of the intestine,
through the systemic effects exerted by it. Studies have shown
increased bacterial diversity in patients with psoriasis compared to
healthy subjects, with reduced Actinobacter colonization and
predominant cutaneous Firmicutes, which may characterize a phenotype
specific to chronic inflammation found in psoriasis. When dysbiosis of
the gut microbiome occurs, microbes and metabolites in the gut induce
regulatory T cell and lymphocyte reactions, which facilitate an
anti-inflammatory response. Studies have also been performed on
translocation of some fragments of transgenic DNA into blood samples
in patients with psoriasis. This suggests that new outbreaks of
psoriasis may be related to the presence of bacterial DNA circulating
in the blood, originating from the intestinal lumen. Healthy
intestinal bacterial composition may reduce intestinal permeability
and the risk of bacterial DNA translocation.
Literature review on the topics covered
In the history of mankind, often after a great discovery, it took many
years for decision makers in that field to accept and promote the
value of discovery. This is what happened with the discovery of the
microbiome with which we are contemporaries. In 1958 the biologist
Joshua Lederber defined the term "microbiome", for which, at the age
of 33, he received the Nobel Prize. Why, for 50 years, the medical
world has not given importance to this discovery, and it was not until
2008 that the European project entitled MetaHIT was launched, which
led to the discovery that people also differ by the bacteria that live
through them, not just by blood type elements.
Dusko Ehrlich, (1) project coordinator at MetaHIT, explains why the
microbiome will play an important role in personalized medicine: ''by
analyzing the genes of the organisms that live in us, physicians can
identify unique aspects of each patient, which will allow conception
of personalized treatments''.
Another microbiologist, Julian Davies, (2) from the University of
British Columbia, states that: "the effort for a better understanding
of the microbiome is the most important scientific project of all
time".
In 2018 researchers from three countries, Belgium, Holland and Malta
wrote a well-documented psoriasis and microbiota: ''Psoriasis and
Microbiota: A Systematic Review'' (3). The purpose of the paper was
that, through a thorough review of the literature, to discuss the skin
and intestinal microbiota and to redefine their role in the
pathogenesis of psoriasis.
1. Following the research, it was concluded that in addition to
improving the course of psoriasis, selective modulation of the
microbiota can increase the effectiveness of medical treatments, as
well as mitigate their side effects. However, no natural modulation of
the microbiome was found.
2. In the paper it is said that in the 1980s, suspensions of the
Malassezia ovalis mushroom fragments applied to the unaffected skin of
patients suffering from psoriasis induced the formation of psoriatic
plaques in all 10 subjects tested (4).
In the same year 1980, being hospitalized with psoriasis in the
Military Hospital, I detached psoriasis shells from my head and body
and placed them under adhesive tape on healthy skin to see what was
happening. Well those placards that looked alive did nothing but
simply dried. Maybe I didn't select those mushrooms mentioned above.
3. The paper also presents a recent study by Darlenski et al. (5) that
provides information on how to improve psoriatic plaques, after
treatment with narrowband ultraviolet radiation (NB-UVB), a
conventional systemic therapy for the treatment of psoriasis.
Treatment with NB-UVB has already been shown to cause significant
changes in the skin microbiota. I also started treatment with
ultraviolet rays. After treatment the skin healed relatively quickly.
But when psoriasis came up again, UV therapy did not work anymore and
then it was used for PUVA rays with the ingestion of Psoralen and
Meladiline pills (photosensitizers). After several sessions for 45
days, the lesions disappeared, but not from the whole body, but only
where there were large surfaces. I agree that after such treatments
with UV or PUVA when the skin is healed and the skin microbiome
returns to normal. But you see, once there are some injuries on the
body, it means that the skin's microbiome is not uniform and in some
places it remains damaged. I personally believe that until the
dysbiosis in the intestinal microbiome is resolved, even if the skin
level is resolved, psoriasis is not cured. It's just that surface
resolution.
4. Regarding the interaction of the microbiotherapy with the
allopathic treatment, it is stated that: ''The skin microbiota also
interacts locally with local treatment in psoriasis''. During the
treatment with Deniplant tea if patients used in combination with
cortisone ointments, at first there was a heal of lesion healing, but
after a short period the lesions returned to the initial stage. In
addition at one point the body seemed to no longer react well to
Deniplant tea because of the cortisone.
My conclusion was that I do not go in parallel. As with treatments
with monoclonal antibodies.
5. In the context of psoriasis, the role of diet (often associated
with exercise) has often been promoted by virtue of its ability to
modulate and improve patients' psoriatic plaques and treatment
effectiveness (6,7).
I also observed in patients who used Deniplant tea and who had
fasting, either at Easter or at Christmas fasting, a faster
improvement of psoriatic lesions, but at the end of the regimen, the
lesions appeared again. If patients permanently change their way of
life (food) is good, because those relapses no longer occur.
If during the treatment with Deniplant tea the patients had other
medical problems and used antibiotics or other drugs, negative changes
were also seen in psoriatic lesions. Any disturbance of the gut
microbiome and the immune system has repercussions on psoriasis
lesions as well.
Theoretical foundation
Recent studies (2015-2016) of Japanese specialists confirm the
importance of the gut microbiome on antitumor immunotherapy and
emphasize the importance of eubiosis (8.9).
The skin microbiome influences the functions of T cells in the skin by
producing IL-2 but also IL-17 (10) and modulates memory T cells (11).
Dysbiosis of the skin microbiome can be caused by changes in the skin
microbiome, alteration of the skin barrier and immune functions, but
also dysbiosis of the intestinal mycobiome.
Since 2010 researchers have been seeking to find oral prebiotics and
probiotics with which to influence the axis of the intestine-nervous
system-skin (12).
Content of the article
As a result of my own experience, during which for 7 years I was ill
with psoriasis, I learned how traumatic this disease can be and what
psycho-emotional implications it can have.
Although we did repeated treatments in the Central Military Hospital,
at first all the lesions disappeared, but as the disease was chronic
and the lesions were on larger areas on the body, even after long-term
treatments, the injuries did not disappear completely.
The fact that the doctors told me that this disease is incurable, made
me look for solutions in herbal medicine and gemotherapy.
After two and a half years of searching and testing on the body, the
medicinal plants and buds of the fruit trees have proved to be the
only effective remedy in my case against the internal causes that
trigger and sustain this disease.
After obtaining a patent for a process for obtaining a herbal extract
for the treatment of psoriasis, we founded the Speranța Medical
Foundation with the aim of supporting the activities of those who know
the mysteries of nature in the field of medicine and their application
for human benefit.
Thus, with the help of scientifically used medicinal plants, the
foundation has shown that certain so-called incurable diseases (for
allopathic medicine) can be ameliorated or even cured. The cases of
cases solved in Romania, and dozens of cases abroad, as well as
obtaining the gold medal at the '97 technical news salon, have proved
this.
In 2005 the natural remedies discovered by me receive a name:
Deniplant - Deniplant brand with the slogan "Health above all" is
registered at OSIM-Romania. Four years later the Deniplant trademark
becomes a Community trademark and the current EUIPO (Office of the
European Union for Intellectual Property) is registered at OHIM.
The use of the natural remedies Deniplant by the patients attending
recovery sessions based on the treatment from Sovata, emphasized that
by intervening on the skin with water and mud from Lake Ursu, the cure
of the disease was much faster.
Seeing these results, we began the study to find an explanation of the
phenomena that occur within the body and which lead to the healing of
these conditions.
Following for one year the courses organized by Prof. Dr. Manole
Cojocaru (13) in which the human microbiome was presented, I came to
the conclusion that these natural remedies discovered by me act on the
human microbiota that influence autoimmune, metabolic and neurological
disorders.
The use of water and sludge from Sovata, salt water from
Călimănești-Căciulata or mud from Techirghiol, allowed the modulation
of the skin microbiome to trigger the reactions needed to heal the
skin.
However, following the patients who used Deniplant tea in parallel
with the aforementioned procedures, we concluded that only modulation
of the skin microbiome cannot definitively solve the problem of
psoriasis, if it does not intervene on the gut microbiome.
The intestinal microbiome triggers the modulation of the skin
microbiome and if it is influenced from the outside, the results can
be amplified (14).
This is also deduced from the fact that in psoriasis when intervening
with ointments or other medicines for external use, the skin
microbiome is affected, but in the long term the changes at that level
can negatively affect the intestinal microbiome and hence those
reactions of rebaund and generalization of the disease.
That is why I think we are facing a new discovery, namely a natural
modulator of the human microbiome.
This year, in collaboration with the Aide-Sante Clinic, we set up a
Biomedicine Center where we are trying to highlight with medical
analysis and evidence this possibility of natural modulation of the
human microbiome with the help of food.
Conclusions
(1) Psoriasis being a common inflammatory skin condition, affecting
about 3% of the world population, must be treated multidisciplinary
and personalized, the microbiome of each patient being a unique entity
that responds in particular to the allopathic and naturalistic
treatment.
(2) The realization of functional foods (nutraceuticals) with a dual
role of nutrition and health, is a desire of both food producers and
those who care about human health, because they can naturally modulate
the activity of the human microbiome, restore eubiosis, the processes
of cellular recovery and healing of the body.
Although it is known how and where prebiotics and probiotics work, it
is necessary to find ways to personalize them according to the medical
condition we want to solve, not being recommended in general.
(3) Future research will focus on the relationship between the human
microbiome and cellular apoptosis in other diseases as well as the
modulation of the intestinal microbiome with the help of dietary
restrictions.
References
[1] Dusko Ehrlich. June 1st, 2011 The next International Human
Microbiome Congress organized by MetaHIT will take place in Paris,
March 19-21, 2012, at the Palais Brongniart.
[2] Psoriasis and Microbiota: A Systematic Review.Diseases. 2018 Jun;
6 (2): 47. Published online 2018 Jun 2: 10.3390/ diseases6020047
PMCID: PMC6023392 PMID: 29865237
[3] Lober C.W., Belew P.W., Rosenberg E.W., Bale G. Patch tests with
killed sonicated microflora in patients with psoriasis. Arch.
Dermatol. 1982; 118: 322-325. doi: 10.1001 / archderm.
1982.01650170036019. [PubMed] [CrossRef] [Google Scholar]
[4] Assarsson M., Duvetorp A., Dienus O., Söderman J., Seifert O.
Significant Changes in the Skin Microbiome in Patients with Chronic
Plaque Psoriasis after Treatment with Narrowband Ultraviolet, B. Acta
Dermato Venereol. 2018; 98: 428-436. doi: 10.2340 / 00015555-2859.
[PubMed] [CrossRef] [Google Scholar]
[5] Roger L.C., Costabile A., Holland D.T., Hoyles L., McCartney A.L.
Examination of faecal Bifidobacterium populations in breast- and
formula-fed infants during the first 18 months of life. Microbiology.
2010; 156: 3329-3341. doi: 10.1099/ mic.0.043224-0. [PubMed]
[CrossRef] [Google Scholar]
[6] Winglee K., Fodor A.A. Intrinsic association between diet and the
gut microbiome: Current evidence. Nutr. Diet. Suppl. 2015; 7:
69-76. [PMC free article] [PubMed] [Google Scholar]
[7] Nishijima S, South W, Oshima K, et al. The gut microbiome of
healthy japanese and its microbial and functional uniwueness. DNA Res
2016; 23: 125-133.
[8] Vetizou M, Pitt JM, Daillere R, et al. Anticancer immunotherapy
by CTLA-4 blockade relies on the gut microbiota. Science 2015; 350:
1079-1084
[9] M. Bentivoglio, P. Pacini, Brain Res. Bull. 38, 161–165 (1995).
[10] H. Takahashi-Iwanaga, H. Shimoda, J. Neurocytol. 32, 363–371 (2003).
[11] Wang Y, Kuo S, Shy M, Yu j, et al. Staphylococcus epidermidis in
the human microbiome mediates fermentation to inhibit growth pf
Propionibacterium acnes: implications of probiotics in acne vulgaris.
Appl Microbiol Biotechnol 2014; 98: 411-424.
[12]
chriskresser.com/do-gut-microbes-control-your-food-cravings/
